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Hemizygous mutant males were generated by crossing heterozygous knockout females to C57BL/6 males.
As specified in the text, control males were generated by crossing Canton S females with males from either the UAS-dTrpA1 or UAS-fruMIR/CyO UAS- frUAS-fruMIR/CyO UAS
Hybrid mdx/Y∶R26/+ males were generated by mating R26 males with C57BL/10ScSn-mdx/J C57BL/10ScSn-mdx/Jing femalesgeny candying both the R26 allele and mdx mutation.
The males were generated by the him-5 e1490) him-5 e1490
Control males were generated by crossing SP,SP +/TM3,Sb,ry males to ∆130/TM3,Sb,ry females to generate SP producing SP,SP +/∆130 (SP+) males.
For all crosses, either males were generated using the method originally described by Sulston and Hodgkin (Hodgkin 2005), or alleles were crossed to males of publically available strains containing integrated fluorescent transgenes that mark distinct nematode structures.
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Males are generated parthenogenetically and females start to produce haploid eggs which must be fertilized.
We find that, similarly to C. elegans, P. pacificus males are generated at low frequencies from self-fertilizing hermaphrodites and are relatively poor mating partners.
A population of 350 half-sib castrated males was generated by mating five males with 400 females and selecting one male offspring per litter, as described in Gallardo et al.[ 7].
Persistence of males in P. pacificus could be explained as a byproduct of the sex determination system, where males are generated by X chromosome non-disjunction events in hermaphrodite meiosis.
Three F0 founder mice, two females and one male, were generated by pronuclear microinjection with the in vivo DNA expression vector, bCN/BChE/hSA.
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