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After the mating period, the males were discarded and the females were transferred to inverted half-pint containers.
Plates harboring any males were discarded.
Males were discarded after 1 week.
The irradiated males were discarded 4 5 days following treatment.
Females were provided with cotton balls moistened with saturated sugar solution; males were discarded.
Afterwards males were discarded and the spermatophore was immediately dissected out of the female's bursa copulatrix.
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These two age classes cover a substantial part of the relevant male ages in this population, since most males are discarded when 4 days old (and males can never become older than 5 days), due to the discrete generation culturing protocol.
Females that had not mated within 2 hr after introducing a male were discarded.
The flies were left together for 2 days, after which the male was discarded, and the females were allowed to lay eggs for an additional 4 days.
Other simple hypotheses of Mendelian inheritance (one or two genes) involving only genetic male sterility were discarded by our data, as they imply that the 'Earlygold' parent should carry alleles conferring fertility in at least one locus.
Last year, documents obtained by the Pesticides Action Network PANN) in Europe found that the EU plans to regulate chemicals linked to cancer and male infertility were discarded after pressure from US trade officials during TTIP negotiations.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com