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Mean longevity of the males were calculated using the raw mortality data and subsequently analysed.
Sperm densities from n = 18 wild and 18 farm males were calculated within 20 h of strip.
To determine whether there is also similarity in probe contribution between M and V libraries, the mean ranks of females and males were calculated for both M and V libraries and then compared with each other.
As a consequence, the expected values for the alpha and beta males were calculated by excluding their maternal relatives as potential mates (e.g. when only one of the alpha male's maternal relative was attractive/peri-ovulatory, the beta male was expected to monopolize her).
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Differences in the mortality rates between scent treated and untreated infected males was calculated using a chi-square test.
The frequency of each cone pigment present in females and males was calculated.
The weight of multiple replicates (minimum of three) of a pool of five females or males was calculated using a precision balance (Mettler Toledo, AG245).
The gene flow of males was calculated as N m m m ≈ [(1 - Fst nucl)/(2 Fst nucl)] - [(1 - Fst mit)/ Fst mit)] by applying Wright's island model [ 83] modified for haplodiploid species [ 84].
2 Briefly, the adjusted G6PD median activity value for normal males was calculated (excluding males with severe G6PD deficiency defined as ≤ 10% of the G6PD median value for all males in the study population), and different cutoff levels of activity were defined (i.e., 10%, 20 %, 30, and 60% of the andusted male median value).
The G6PD quantitative spectrophotometric assay from capillary and venous blood was used as the reference assay; the G6PD median value for normal males was calculated using the data from 26 G6PD-normal males, and the respective 20%, 30%, and 40% thresholds were used to assess performances of qualitative tests.
The relative strength of gene flow through females or males was calculated according to Seppä et al. [ 44], with the effective gene flow of females estimated as N f m f ≈ (1 - Fst mit)/(2 Fst mit) [ 82], where N f is the number of females breeding in a population and m f the immigration rate.
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