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Males were added to the cages after the second injection.
Each hermaphrodite was then moved to an individual plate, to which four 1-day-old adult N2 males were added.
When two healthy, nonexposed males were added to the populations that had experienced reproductive failure, embryos began surviving.
In the rainbow trout (Oncorhynchus mykiss) the first male sired over 75% of the eggs when sperm of four males were added in 30s intervals [ 13].
At artificial dawn, individual males were added to 1.5 L plastic pots containing 6 females, except for control males which were placed in empty 1.5 L pots.
For each replicate, males were added to a drop of water on a microscope slide printed with 10 6-mm diameter wells.
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Females were placed into vials first, and then the appropriate number of males was added.
After a vicious costly civil war, the word "male" was added to the constitution to allow black males -- but not black females -- to vote.
The next day, within one hour of the incubator lights on, a single 4-day old male was added to each vial.
According to Mulder et al. [ 25], the number of genotyped ancestor generations affects the accuracy of genomic predictions.> -wrap-foot> aGenomic parent average included genomic information on both sexes; validation was done in both sexes For males in the validation population, accuracy improved significantly when male genotypes were added to the training population (Fig. 3).
To induce sexual spheroids, 0.1 0.2 mL of the sexual inducer (supernatant of the male culture after the formation of sperm packets) and ca. 0.5 mL of an actively growing female or male strain were added to 11 mL of fresh VTAC medium in a tube and placed at 25 °C with a 14 10 h LD cycle.
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