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Female preference for conspecific P. emmiltos males was maintained under monochromatic light, but lost when non-visual cues were blocked (response variable is the number of replicates in which the majority of the clutch was laid next to a male of a particular species).
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This situation sets up the prospect for frequency-dependent selection maintaining an equilibrium, wherein both types of males are maintained at more or less constant frequencies.
Males were maintained on 12 h day and 12 h night cycle.
However, normal fertility in the exposed males is maintained because of the intact DBY gene in the germline.
Naive males were maintained in isolation until their first contest.
Males were maintained using small mating plates with 7-8 mands and 2 adult hermaphrodites.
Males were maintained in individual cups (250 ml) on a diet of Drosophila.
For the long term mating assays, males were maintained throughout the female lifetime.
Experienced males were maintained in identical conditions to naive males until 3 days before their first contest.
In our model, with K = 10, about 1000 individuals (500 males) were maintained in the 1000 × 1000 cells habitat.
Males were maintained on individual plates to avoid male-male interactions that influence lifespan; hermaphrodite lifespan was not affected by singling animals ([ 15] and data not shown).
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