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On 2nd day after eclosion, virgin selection line males (from one of the 9 populations) were combined with virgin LH-females (5 males: 5 females) in vials with standard food and supplemented with live yeast (2.33 mg per vial).
Finally, we consider a case in which males from one population in (S1, S2, H) are compared to females from a different population.
Therefore male introgression would be the most likely introgression scenario, where males from one taxon invade groups of a neighbouring taxon and reproduce successfully.
Interpopulation crosses were carried out between randomly picked males from one replicate population and randomly picked females from another replicate population of the same size.
When 3 days old, each group was transferred to a fresh vial containing 10 equivalently aged males from one of the five strains, with all mitochondrial strain × laboratory strain cross-combinations carried out.
We conducted short-term adult survival and fecundity trials by placing 50 males from one line (used in the previous attractiveness measures) and 50 naive virgin females from the same line into a 250 litre mating tank.
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Females ranged from one-eighth to half an inch long, males from one-sixth to a third of an inch.
He offered advice to Pale Male, from one homeless New York icon to another: "Defecate on the tenants as they walk into the building.
Every male from one month old and up.
Fifth, after approximately 50 genotypes, we genotyped our first white-eyed opa23 male from one of the F2 crosses.
"Females" from strain JK574 were mated sequentially to two males: a PD4790 reference strain marked phenotypically with the genetically dominant, pharyngially-expressed green fluorescent protein (GFP), and a male from one of six wild isolate strains (Table 1).
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