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Evidence of FBD in T. tana was also provided by significantly lower relatedness values among adult females than among males for two pairwise measures of relatedness.
F0 injC females were then crossed with asymbiotic males for two generations in order to produce two year old F2 virgin injC females.
As predicted for FBD, adult females had significantly lower mean values than males for two different tests (mAIc and pairwise relatedness).
Using McGregor & Krebs' study [17] as an example, we performed a longitudinal analysis following colour-ringed males for two years to determine whether repertoire size and/or composition change between years in adult males.
Therefore, the virus cannot be transmitted through males for two successive generations.
Therefore, this virus cannot be transmitted through males for two successive generations.
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He was going on puppy paternity leave, which, his wife told me, was "not a request": she had potty-trained enough males for one lifetime.
Females were caged with males for one night and pregnancy was detected by abdominal palpation after 14 days.
On the evening of mating, females were placed with singly housed wild type males for three hours (17 30 20 30) and then checked for copulation plugs.
We also used a G-test of goodness-of-fit to compare the proportion of female visits to courting males for six days at each area under low and high predation risk.
Briefly, interspecies F1 females were backcrossed to D. p. bogotana males for ten generations to purge the background of D. persimilis alleles at regions other than those coinciding with the focal Q2 QTL.
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