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Frogs were weighed (nearest 0.1 g), snout vent length (SVL) measured (nearest mm), and males assigned to one of two size categories, large (SVL >52 mm) or small (SVL <50 mm).
Males assigned to other recovery periods were moved individually to 500 ml plastic pots lined with a moist cotton pad and provided with ground corn until the appropriate time of dissection.
For the post-male competition experiment, both males and female were immediately returned to the first experimental tank, with the competing males assigned to the opposite compartment from the pre-male competition experiment.
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The male assigned to the first mating to be allocated to a MOET female has to be used for all her remaining matings.
The 64 males were also tested twice; however, males were assigned to new pairs for the second trial.
All females, but only 35 of 42 males, were assigned to offspring, likely because the sperm from seven males was not activated or the fertilized eggs were not selected for in the experiment.
One reason may be because the females in Karakal volunteered specifically for service in Karakal while the males were assigned to the unit from the general pool of males designated for combat service.
Virgin males were assigned to mate with several virgin females in succession.
One-day-old BUT Big-6 males were assigned to six weight-matched groups.
Ninety-eight percent of males were assigned to a single cluster with greater than 95% posterior probability.
In lingual mandibles, 97% of males were assigned to one cluster with greater than 95% posterior probability, while 96% of females were assigned to a different cluster also with greater than 95% posterior probability.
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CEO of Professional Science Editing for Scientists @ prosciediting.com