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Both cauda epididymides from a single male were placed in a 900 µl drop of human tubal fluid (HTF) medium [29] supplemented with 0.5% BSA.
In each replicate, one female and one male were placed on the lower surface of a plant leaf (third to fifth leaf from the top) enclosed in a clip-cage.
To examine the role of chemical signalling in male parental experience, the above protocol was repeated but following the initial female choice for a male, the female and the chosen male were placed in separate crystallizing dishes containing seawater.
One virgin female and one virgin male were placed together in a food vial.
Two females and a male were placed together for mating in one tonne fibre re-inforced plastic (FRP) tanks.
Single pairs comprising an F1 female and an F1 male were placed in separate petri plates containing 12 ml of culture medium.
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After 5 days of conditioning, one male was placed in a green or tilted box for 5 minutes, and another male was placed in an unfamiliar box.
If no copulation occurred within 1 hour, a new male was placed in the container for a second hour.
A fluorescein-injected male was placed in an 80-L aquarium marked lengthwise into four 12.5 cm wide quadrants.
The rejected male was placed in another crystallizing dish containing 'conditioned water' taken from a male that was undertaking egg care.
In Drosophila, when a male is placed in an experimental arena with a single female present, robust courtship behavior ensues [50], [51], [52].
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