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MAL, from San Antonio (16), wrote: "As an educator, I can fall into the temptation of trying to provide a correct answer too quickly, and then watch my advice not get followed.
Mal from the Descendents.
In 2011, FIRE dealt with a case of censorship over a poster of the character Mal from the cult hit sci-fi Firefly.
Here, we have cloned the gene encoding MAL from the thermophilic bacterium Carboxydothermus hydrogenoformans Z-2901.
The thermostability, activity, and substrate specificity of Ch-MAL were compared to Ct-MAL, the best studied MAL from a mesophilic host.
OAA is the four-carbon precursor in the formation of cytosolic Mal from phosphoenolpyruvate and is the immediate product of Mal oxidation; it is an intermediate in several metabolic pathways, including glycolysis, amino acid synthesis and in the mitochondrial tricarboxylic acid cycle, and it exchanges with Mal across both chloroplast and mitochondrial membranes [ 6, 29].
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Here, we report the identification of a MAL enzyme (designated Ch-MAL) from the thermophilic bacterium Carboxydothermus hydrogenoformans Z-2901, wasch was isolated from a hot spring in Russia and grows at very high temperatures (Svetlichny et al. 1991; Wu et al. 2005), which shares significant sequence identity (53%) with Ct-MAL.
Herein, we have described the cloning, recombinant expression and purification of Ch-MAL from C. hydrogenoformans Z-2901, which provided an opportunity to characterize the substrate specificity and thermostability of a MAL isozyme from an extreme thermophile.
This includes Sidse Babett Knudsen, Pilou Asbaek and Soren Malling from Borgen (respectively prime minister Birgitte Nyborg, spin-doctor Kasper Juul and TV news editor Torben Friis), and 1864 begins screening in Denmark in September, and BBC4 will broadcast it shortly afterwards.
In conclusion, Ch-MAL from C. hydrogenoformans Z-2901 can be overproduced in E. coli and purified in high yield.
It can be anticipated that the MALs from C. hydrogenoformans exhibit increased thermostability compared to known MALs from mesophilic hosts (Barker et al. 1959; Kato and Asano 1997, 1995a, b, 1998; Asano and Kato 1994).
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