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Because the linearized vector is not gel purified, it is important to ensure by checking the digests that the vector digestions contain only linearized molecules any remaining uncut supercoiled vector molecules would transform competent E. coli with too great an efficiency, making transformation with the recombinant plasmid less likely.
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We all make transformations and changes.
Hong Kong made the transformation with scarcely a dent in the economy's near-full employment, and with a huge leap in general prosperity.Two new books on Hong Kong's economy, "The Hong Kong Advantage"* and "Made by Hong Kong"†, suggest that the territory is well placed once again to adapt to changing circumstances.
Making this transformation – and it is a transformation – requires painstaking work and it takes time.
Chairman Bob Lawson insisted "good progress" was being made towards the transformation, with the latest results underlining the benefits of concentrating on a single core area.
In the frequency domain, we design an α-DoB band-pass filter to conduct coarse saliency detection and make an amplitude transformation with smoothing processing which is the so-called elaborate saliency detection.
All strains and plasmids are listed in Table 2. B. subtilis cells were made competent for transformation with DNA either by the method of Kunst and Rapoport [41], or by the method of Anagnostopoulos and Spizizen [42] as modified by Jenkinson [43].
These factors are making the transformation into a more reformist governance, slow, filled with hurdles and punctuated with intense bloodshed.
This crew was actually capable of making this transformation happen.
I'm really glad I am making this transformation.
Our pilot mapping experiment shows that plummeting sequencing costs make natural transformation combined with genomics a powerful means to map natural phenotypic variation.
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