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These measurements utilize fluorescence (de quenching and FRET assays performed in 384-well microplates, making the assays suitable for high-throughput characterization of interactions at low concentrations.
The data from the multiplex real-time PCR assays are obtainable only 30 min after DNA extraction of the samples, making the assays useful for screening samples containing DNA from unknown animal origin in the forensic field.
We then performed soft agar colony formation assays using a CytoSelect™ 96-Well In Vitro Tumor Sensitivity Assay Kit, which can be used to count the colony-forming cells by means of a colorimetric method, such as the WST-8 assay, making the assays quick and accurate.
High-throughput yeast two-hybrid assays also require extensive liquid handling infrastructures [ 5, 24] making the assays less accessible for many research groups.
For some assays, inclusion of breast cells or proteins isolated from them may be important prerequisites to making the assays relevant to breast cancer.
By making the assays that test different aspects of memory and learning more comparable, Vogt, Schnaitmann, Tanimoto and co-workers have now placed Drosophila researchers in a better position to understand how the fly brain makes these decisions.
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These peptides can be employed for rapid detection of C. albicans by a simple ELISA based assay which allows testing multiple samples at the same time and thus making the assay high-throughput.
Full automation of the analysis, with emphasis on making the assay amenable to high-throughput screening, is in progress.
The procoagulant activity of meizothrombin is low, amounting to approximately 10% of the equimolar thrombin mass, making the assay less sensitive than a direct assay of thrombin activity.
This combination reacts with both normal and phosphorylated tau with epitopes in the mid-region of the molecule, making the assay sensitive to all known tau isoforms.
The detection of DENV using RT-LAMP regardless of the viral load could be completed within 1 hour making the assay a rapid tool for early dengue diagnosis.
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