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Acceptor photobleaching-FRET (AP-FRET) was measured as described in [18] by making necessary settings in a Zeiss LSM 510 confocal microscope with a C-Apochromat 63×1.2 W objective.
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The phased implementation provided an opportunity for learning and adapting the program to local settings and making necessary changes.
Making necessary adjustments to equipment.
Industrials do make necessary things.
Make necessary changes.
Make necessary repairs.
Make necessary adjustments if required.
Some capitalists make "necessaries," some make "luxuries".
Methods of allocating water are sensitive to physical, social, institutional and political settings, making it necessary to design allocation mechanisms accordingly.
It is necessary to make your settings higher.
The recognition of differential expression of the multiple Nox isoforms in the vessel wall, their varying regulation, and their differential expression in disease settings make it necessary to assess the contributions of individual isoforms in different locations.
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