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The mechanisms of protein aggregation vary depending on the protein and the expression system utilized, making it difficult to elucidate the conditions that promote their formation.
However, as described above, limitations still exist, making it difficult to elucidate the functions of lncRNAs overlapping with other genes and stimulate lncRNA expression specifically (Quinn and Chang, 2016), which could be overcome by our CTRL system.
Sharks can be detected ≤1000 m from each other making it difficult to elucidate information regarding individual interactions, such as social behaviours, predator prey encounters and courtship/mating events.
Furthermore, OC lacks a clear molecular profile making it difficult to elucidate a universal cause in OC initiation or progression [ 41– 41].
However, these cross-sectional studies were performed following long-term joint pathology, making it difficult to elucidate the effects of joint injury from other factors such as disuse, medication use and varying rehabilitation programmes.
Although many studies have investigated mRNA from muscle biopsies, making it difficult to elucidate the cellular source of the chemokines, there are data that IIM muscle fibers themselves can produce chemokines.
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However, the nanostructure density, in such samples, makes it difficult to elucidate the exact growth mechanism.
First, the retrospective nature of this study makes it difficult to elucidate known confounders that could have biased the outcome measures, such as differences in sepsis treatment.
A lack of the understanding of plant acclimation to future climate change makes it difficult to elucidate the role of the forest in global carbon cycle on a long time scale.
Over-expression of various CBF/DREB genes in diverse plants have been reported, but inconsistency of gene donor, recipient genus, parameters used in evaluating the transgenic plants have made it difficult to elucidate the complex physiological mechanisms by which DREBs impact drought tolerance.
This makes it difficult to elucidate the detailed functional mechanism of aspirin in relation to bone remodeling.
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