Sentence examples for making direct sequencing from inspiring English sources

However, some organisms exhibit no intra-genomic variation, making direct sequencing possible [ 54, 63, 69, 73].

The ITS1 region was often variable within a species and even within one animal, making direct sequencing of PCR products problematic (data not shown).

The large amount of sequencing data that can readily be produced by next-generation sequencing platforms, such as the Illumina GAII, reduces the need for prior sequence knowledge for gene expression profiling and are now making direct sequencing approaches the method of choice for whole transcriptome analysis in many species [ 22- 26].

Although molecular mimicry has been hypothesized as a possible trigger of autoimmunity in NMDARe, the epitope targeted by the autoantibodies appears to be a conformational epitope, making direct sequence comparisons to known pathogens difficult.

In these two plastid-targeted genes the different sequences are heterogeneous at the amino acid level in the signal peptide and chloroplast targeting sequence, and the length variation in this region made direct sequencing difficult.

Furthermore, the presence of multiple distinct sequences would make direct sequencing impossible.

The concerted evolution of the two copies of IR is complete, which makes direct sequencing possible without cloning.

Hsp53 makes direct sequence-specific contacts with bases in the major groove of DNA via the side chains of K120, A276, C277 and R280.

The effects of alignment size bias are especially noticeable for alignments containing fewer than about 100 sequences, and must be accounted for when making direct comparisons between sequence alignments of different sizes.

Additionally, group- and taxon-specific oligonucleotide probes can be developed from these sequences making direct visualization of microorganisms in soil habitats possible.

The ability to sequence unrefined PCR products and the requirement of only 4% of the AFP makes direct end sequencing of AFP an effective means of verifying array spotting solution.