Sentence examples for making conditional from inspiring English sources
People are putting much more effort into making conditional alleles with CRISPR rather than making ES cells".
Because CRISPR is less adept at making conditional knockouts, William Skarnes, who led a team making mutant mouse ES cells at the Wellcome Trust Sanger Institute in Hinxton, U.K., worries that NIH is overemphasizing the new approach.
Using CRISPR to insert this same system into zygotes, Jaenisch's team reported making conditional mice with relatively "high efficiency"—about 16% of the zygotes led to mouse pups with the correct mutations.
During the process of making conditional knockout in human colon cancer cell line HCT116, while no removal of Neo cassette was observed in 24 clones carrying the FLPe transgene (either under CAG or CMV promoter), 100% clones carrying the FLPo transgene (under PGK promoter) become G418-sensitive (unpublished data).
Once genes have been selected for testing, there are a number of options for allele selection, including making conditional knockout alleles, or obtaining gene-trap or targeted allele embryonic stem cells from distribution centres.
Further, more complex genome engineering applications such as the incorporation of recombination sites for making conditional alleles, endogenous protein tagging, or precise editing of genomic sequences require HDR (Golic 2013; Gratz et al. 2013b; Bassett and Liu 2014; Beumer and Carroll 2014).
The act was made conditional on its renewal by December 1970.
Most have made conditional endorsements, perhaps reflecting the political divisions the hunt has created among residents.
Release of the tax returns should not be made conditional on winning the nomination, as Senator Hillary Rodham Clinton has made it.
By contrast, 80percentt had made conditional free shipping offers, and 71percentt said they would use this approach more this year.
At the same time, support for disabled people is being slashed and made conditional on humiliating tests to discourage applicants.
