The resulting degree of homology makes sequencing and alignment in this region particularly challenging, with the problem particularly acute for IL28A and IL28B, which share identity for 1309 bases over a 1339 bp length.
Interferometry requires the use of multiple objective lenses, significantly increasing the complexity of the system and making alignment and data processing more challenging.
BBO sources however have the advantage of using much cheaper crystals, as well as the ability to manufacture crystals with large apertures, making alignment and collection of downconverted light much simpler [28].
Large genome sizes, an abundance of repeated sequences, and polyploidy present challenges to genome-wide SNP discovery using next-generation sequencing (NGS) of total genomic DNA by making alignment and clustering of short reads generated by the NGS platforms difficult, particularly in the absence of a reference genome sequence.
It is a noncoding region that evolves fast and makes alignment and analysis difficult beyond quite small taxonomic distances.
Successful 21st Century Organizations can reformulate their work environments into Breakthrough Performance Environments environments which flex to support the rapid movement, speedy decision-making and alignment required to outperform competitors and regularly achieve extraordinary outcomes.
There is room in the Christian Church for conservatives and liberals, and I am glad to be part of a Presbyterian denomination that finds its vitality in democratic decision-making and alignment between clergy and laypeople on most important issues.
Ensuring and making clear the alignment between principles and purpose of the gesture, and its inherent power (such as financial incentives, regulations or sanctions), may influence the responses from which will emerge changing patterns of organizing.
This makes alignment and analysis difficult, and the interpretation of these data should be done with caution.
The junctions between the repeats in the 1.8 kb insertion are also impossible to align, suggesting a high mutation rate associated with duplication and chimera formation that makes alignment and measurement of the rate impossible.
