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After making a "scratch" in the cell layer with a 200 μl pipette tip, CPC cultures were rinsed gently to discard debris.
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"Didn't even make a scratch," Sanchez told me.
But in her third-round match Friday against Wimbledon semifinalist and No. 22 seed Sabine Lisicki, Falconi was unable to make a scratch.
After a stainless steel sample is polarized in the passive part of the anodic polarization curve, a sharp pin makes a scratch on the surface, hereby removing the passive film.
As a reviewer, it's my job to at least make a scratch at describing the plot of the novel, but as a fellow novelist, I balk at giving more than a few bare details.
It's way too early to say if DotSpots can make a scratch on that.
Driving towards the peninsula's tip, it's clear that human settlement has hardly made a scratch on this barren landscape.
What Ed Miliband proposed yesterday doesn't even make a scratch on the number of people who are in poverty despite being in work.
A pipette tip was used to make a scratch wound, then the cells were transferred to media supplemented with 10 mM HEPES (Mediatech, Manassas, VA) and mounted in a heated chamber (Warner Instruments, Hamden, CT) to maintain cells at 37°C.
Cells were then plated onto collagen I coated dishes for 2 hours, thereafter, a sterile pipette tip was used to make a scratch in the cell monolayer and non-adherent cells were removed by gentle washing.
A 10 μl tip was used to make a scratch in the confluent layer of cells.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com