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The α-cyano-4-hydroxycinnamic acid matrix was applied: the matrix solution was prepared by making a saturated solution of the matrix powder into TA30 solution (30% ACN and 70% water containing 0.1% TFA).
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The amount of solute required to make a saturated solution at a given condition is called the solubility.
To use it, mix the crystals with hot water to make a saturated solution (keep adding crystals until no more will dissolve in the container of water).
Just enough of the acid was added to dissolve the precipitate to make a saturated solution of the salt.
The cell culture was incubated O/N at 30°/220 rpm using a Multitron Infors Incubator and then mixed with 100 µL of 50% glycerol to make a saturated library stock.
Because the CMC of oleic acid is below the concentration needed to make a saturated MSI1 sample, we cannot form sufficient MSI1-oleic acid complex for NMR structural studies.
This will make a saturated solution of alum.
Finally, there is an underlying question about whether these two media organizations were driving too hard to make a splash in today's saturated market, a frenzy that perhaps led to cutting corners and hyping the piece.
Suntory, which sells a range of soft drinks in Japan including Pepsi, Orangina and the Boss canned coffee line, has been expanding overseas to make up for a saturated market back home.
Whereas previous generations only had Melrose Place Matt (whose very kiss made the screen fade to black) or Eponymous Ellen (whose groundbreaking tropes were so media-saturated that it almost made a focused conversation impossible), current students have a pantheon of queer and ally characters to reference and apply to dialogue, which is a professor's dream.
The precision studies were performed by making 136 punches from a saturated 15.0 cm filter.
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