Sentence examples similar to making a construct from inspiring English sources

However, limited enzyme sites and increased insertion size lower the efficiency to make a construct.

We made a construct containing eight anthocyanin-related genes (two regulatory genes from maize and six structural genes from Coleus) driven by the endosperm-specific promoters,plus a selectable marker and a gene for marker excision.

We chose to make a construct containing a GFP gene with 4 introns and 5 exons (the same construct as described previously [21] but with introns).

To test this hypothesis, we made a construct coding for a non-HA tagged version of the 38 aa TLR4 transmembrane domain.

To further explore the putative IRES function of the Ring1B 5' UTR, we also made a construct with a small (about 140 bp) synthetic stem-loop-stem structure 3' of the transcription start site but 5' of the IRES (figure 2A, construct nr. 1).

A major aim in this initial work was to make a construct that was highly responsive to oestrogenic EDCs.

To test the first hypothesis, we made a construct expressing the peptide from Arg to Asp on human α1 as a GST fusion.

We then made a construct that expresses only the first 640 amino acids of CSPG4 (CSPG4-N1-640, Figure 3A), and the Fc-tagged CSPG4-N30-640 was pusinged using protein G agarose.

Grover and Roughley [ 55] made a construct containing the Col2a1 promoter driving rtTA that undergoes a conformational change, allowing it to bind to the tetO element that was present within the same construct, coupled to a minimal cytomegalovirus (CMV) promoter.

We made a construct containing the full Saccharomyces cerevisiae Smc3 ATPase domain with the coiled coils and hinge replaced by a short flexible linker (Supplementary Figures 2c and d) to create a monopartite ATPase head (ScSmc3AH).

To determine whether loss of the last exon of FGFR3 is functionally relevant, we also made a construct in which a stop codon was introduced at the end of wild-type FGFR3 exon 18 (del18).