Sentence examples for makes this assay from inspiring English sources
The broad generic nature of the PCR primers coupled with the enhanced sensitivity and specificity of the fluorescent hybridisation probes makes this assay potentially valuable for both routine diagnostic and epidemiological work.
Additionally, the use of banked cryopreserved PBMC samples makes this assay attractive in the setting of large efficacy trials where it is less feasible to perform immunoassays on freshly isolated samples.
However, the additional capability of the new assay, namely the ability of not only excluding but also quantifying 3-epimers of D2 and D3, along with the primary forms (D2 and D3), 1,25α(OH)2D3 and 1,25α(OH)2D2, makes this assay instrumental in research and clinical practice where specific and accurate measurement of the different forms is required.
Because a PCR amplification step makes this assay extremely sensitive, it may provide the basis in the future for standardized clinical SH2 profiling assays for molecular diagnostics.
The ability of real-time RT-PCR to test the expression of very small mRNA fragments makes this assay affordable for studies with these kind of samples, in which the RNA is moderately or even highly degraded, and yet it still produces reliable results [22], [23].
This technique, considered the gold standard for measurement of gene expression, has the ability to analyze very small mRNA fragments makes this assay feasible for studies with formalin-fixed paraffin-embedded samples, in which the RNA is moderately or even highly degraded.
Refinements in the probe design and data analysis software will increase the analytical sensitivity of insertions and deletions making this assay even more applicable for clinical testing.
These results demonstrate that GFP− and GFP+ cells accurately reflect the presence of expansions and contractions, making this assay especially well suited to detect expansions and contractions quickly within a chromosomal environment.
Features of this assay such as high throughput, cell number requirement and cytokine choice should make this assay a valuable tool for screening for HIV-specific immune responses in several clinically relevant settings.
As a result, we suggest a new RQ-PCR procedure that is based on Taqman probe technology and that takes into account the PCR inhibition problems, making this assay more reliable in a routine molecular laboratory.
Loop-mediated isothermal amplification (LAMP) has been shown to be simple, rapid and cost-effective, characteristics which make this assay suitable for viral load monitoring in resource limited settings.
