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The PCR multiplexes amplify short amplicon lengths which makes the assay sensitive to the analysis of degraded DNA.
A very good leaving group (pKa of DiFMU ∼ 4.7) in the phosphate monoester substrate makes the assay highly sensitive.
The lack of a suitable model for global culture behavior makes the assay of new methodologies a costly and tenuous task.
The antibodies showed negligible cross reactivity (CR) with other OP toxicants and pesticides, which makes the assay suitable for the selective detection of sarin.
Top reading of the nascent fluorescence makes the assay very convenient with no need to separate the particulate adjuvants from the reaction mixtures.
Moreover, this assay related DNA probe does not involve any modification and the whole assay proceeds in one tube, which makes the assay simple and low cost.
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The sensitivity and the small sample requirements make the assay suitable for high-throughput screening of biological samples.
Therefore, The proper primers was designed as close as possible to the STRs region to produce smaller size STRs, and made the assay suitable for the destroyed samples.
In order to make the assay more accessible to a resource-limited clinical setting, we eliminated the ELAST system, which simplified the Up24 assay, reduced its cost, and tested the accuracy of the modified assay in a rural Malawian hospital.
This modification made the assay less susceptible to artifacts and prevented overestimation of antioxidant power (Huang et al. 2013).
The next step is to make the assay reproducibly quantitative.
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CEO of Professional Science Editing for Scientists @ prosciediting.com