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Species in the genus Lilium have huge genomes (around 36 GB), which makes sequencing and assembly of their genomes a technical challenge (Du et al. 2017).
The rapid evolution of knowledge about the genetic foundations of tuberculosis drug resistance makes sequencing a versatile technology platform for providing rapid, accurate, and actionable results for treating this disease.
Taken together, this makes sequencing less laborious and more economic.
The polyclonal nature of many P.falciparum infections [53] complicates gene counting and makes sequencing an unreliable method for estimating gene copy numbers.
Sequence similarity makes sequencing of single genes and allocation of sequences into respective genomes difficult.
Flanking of the polylinker with T7 and SP6 universal primer sites makes sequencing of any insert straightforward.
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This makes sequence-level adaptation unsuitable for real-time applications, or for network applications, where dynamic modeling of video data traffic is required.
This makes sequence analysis very difficult.
This makes sequence-based virus classification more feasible.
However, "next gen" sequencing is designed to use many small pieces of DNA, and so has made sequencing older DNA much easier.
Continued advances in DNA sequencing technology will make sequencing a routine biological assay.
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