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This makes sequence analysis very difficult.
Strawberry has unrealized potential as a research model and tool, and the lack of molecular markers for breeding and the eventual need for genetic improvement of the current suite of cultivars makes sequence examination especially timely.
The 454 pyrosequencing method uses randomly sheared DNA, has no requirement for known primer sequences (making it suitable for de novo sequence surveys), and makes sequence data faster and cheaper to obtain than Sanger-based methods.
This makes sequence identification possible only at the genus level or above and what may be newly discovered diversity is often discarded as we are unable to confidently place sequences in the 'Tree of Life' [ 61].
PAX proteins are defined by the presence of an 128 amino acid DNA binding domain at their amino terminal end referred to as the, 'Paired Domain', which makes sequence specific contact with DNA and regulates the transcription of select genes.
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This makes sequence-level adaptation unsuitable for real-time applications, or for network applications, where dynamic modeling of video data traffic is required.
In the wild-type enzyme Asp235 makes sequence-specific hydrogen bonds through its side chain carboxylate group with base pair G3.C70 in the minor groove of the acceptor stem of the tRNA.
Species in the genus Lilium have huge genomes (around 36 GB), which makes sequencing and assembly of their genomes a technical challenge (Du et al. 2017).
PAX proteins are defined by the presence of a DNA-binding domain, the 'paired domain', which makes sequence-specific contact with DNA.
Taken together, this makes sequencing less laborious and more economic.
This makes sequence-based virus classification more feasible.
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