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The solutions were quantitatively transferred to 10 mL volumetric flasks and each flask was made up to volume with methanol.
Master equimolar solution of gabapentin was prepared in water whereas NIN was prepared in methanol and made up to volume with the same solvent.
It was filtered using Millipore water purification System (New Orleans, USA) and filtrate was made up to volume 10 mL in volumetric flask with HPLC grade methanol.
Aliquots of this standard solution were transferred using A-grade bulb pipettes into 10-mL volumetric flasks, and the solutions were made up to volume with mobile phase to give final concentration of 10 μg/mL.
After centrifugation, the combined solution was transferred into a 25-mL volumetric flask and made up to volume with 70% methanol and filtered through a syringe filter (0.2 μm, Alltech, Beerfield, IL, USA).
The samples were digested in a 1 2 (v/v) mixture of nitric and hydrochloric acids in PDS-6 pressure digestion systems (Loftfields Analytical Solutions, Neu Eichenberg, Germany) and were then made up to volume and mixed, and impurities were pelleted by centrifugation.
The stock solution was protected from light using aluminum foil and aliquots of the standard stock solution of GLM were transferred using A-grade bulb pipettes into 10-mL volumetric flasks and the solutions were made up to volume with mobile phase to give final concentrations in the range of 0.2, 0.4, 0.8, 0.9, 1.2, 1.4, and 2 μg/mL.
The whole content was allowed to stand for 5 min. The solution was then filtered through a cotton wool plug into a 10-mL volumetric flask and made up to volume by adding fresh glacial acetic acid rinsing the filter aid in the process.
The solutions were heated in thermostatically controlled water bath at 70 ± 10°C for 20 min. The reaction was stopped by cooling under tap water, and then 0.2 mL of HCl was added and the solutions were made up to volume with distilled water.
The solutions were heated in thermostatically controlled water bath at 60°C for 15 min. The reaction was stopped by cooling under tap water, then 0.2 mL of concentrated HCl was added and the flasks were made up to volume with water.
The solutions were heated in thermostatically controlled water bath at 70°C for 25 min. The reaction was stopped by cooling under tap water, then 0.2 mL of concentrated HCl was added and the solutions were made up to volume with methanol.
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The phrase "make up to volume " functions as an instruction, typically in scientific or technical contexts, indicating the action of increasing the quantity of a substance, usually a liquid, until it reaches a specific, predetermined volume. Ludwig AI highlights the phrase's grammatical issues.
Expression frequency: Missing
