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Exact(7)
Features of this assay such as high throughput, cell number requirement and cytokine choice should make this assay a valuable tool for screening for HIV-specific immune responses in several clinically relevant settings.
Loop-mediated isothermal amplification (LAMP) has been shown to be simple, rapid and cost-effective, characteristics which make this assay suitable for viral load monitoring in resource limited settings.
Improvement in the sequence of the primers and probes in order to correctly classify HCV subtypes 1a and 1b is underway in order to make this assay a useful tool for clinical trials and practice.
The strength of the Renilla luciferase signals make this assay perfectly suited for screening factors required for fusion.
To make this assay more reliable to clinical standards, any SMs detected as "lower confidence" were considered undetected.
Currently there is no validated PCR test in common use, only in-house systems which are open to differing interpretation and none would meet the rigors of quality control to make this assay used worldwide.
Similar(53)
Although SCGE assay has its some advantages and disadvantages, yet some modifications made this assay useful, reliable and consistent for variety of forensic applications.
Refinements in the probe design and data analysis software will increase the analytical sensitivity of insertions and deletions making this assay even more applicable for clinical testing.
The broad generic nature of the PCR primers coupled with the enhanced sensitivity and specificity of the fluorescent hybridisation probes makes this assay potentially valuable for both routine diagnostic and epidemiological work.
Additionally, the use of banked cryopreserved PBMC samples makes this assay attractive in the setting of large efficacy trials where it is less feasible to perform immunoassays on freshly isolated samples.
As a result, we suggest a new RQ-PCR procedure that is based on Taqman probe technology and that takes into account the PCR inhibition problems, making this assay more reliable in a routine molecular laboratory.
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