Exact(3)
The resulting isolate was analyzed with the ansB-Rev-Far downstream primer that lies outside of all of the primers used to make the mutation construct along with the sacBSCN-F2 primer to ensure proper insertion of the cassette into the chromosome.
In the current version we have modified the headings for each plot to make the mutation type clear without making reading the figure legend necessary.
Query 3: 'Find all IKMC mice available from the EMMA Repository with information on the vector used to make the mutation'.
Similar(57)
The intergenic spacer (IGS, Figure 1) is internally repetitive making the mutation analysis of this region difficult.
However, fitness differences would reduce frequency of deleterious alleles, making the mutation rate appear lower than it actually is.
From these results, an interesting observation can be made: The mutation causes more extreme base composition in the evolving genotypes, making the genotypes of different species distinct.
DOI: http://dx.doi.org/10.7554/eLife.01213.009 To test further our prediction that the carbamylated K125 forms a salt bridge with R104, we made the mutation K125E in Cx26.
Although it is typically quicker to introduce mutations into a plasmid-borne copy of a gene than into the genome, there are several advantages to making the mutation in the genome, particularly when measuring the effect of these mutations on gene expression.
The function of the 3′ region of the ZRS became obvious after making the mutations at the endogenous locus.
The mutations make the channel, called NaV1.7, more easily blocked than it usually is.
In addition, the mutations make the inflorescence transcriptome less similar to mature pollen and seed transcriptomes.
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