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The sensitivity and the small sample requirements make the assay suitable for high-throughput screening of biological samples.
In order to make the assay more accessible to a resource-limited clinical setting, we eliminated the ELAST system, which simplified the Up24 assay, reduced its cost, and tested the accuracy of the modified assay in a rural Malawian hospital.
The next step is to make the assay reproducibly quantitative.
To make the assay robust for clinical use a chemical compound was applied to the samples that inhibited interference from the classical pathway due to the presence of anti-BSA antibodies in some sera.
Those features make the assay system ideal for systematic investigation of cellular/molecular mechanisms underlying the pMV-absorption, namely structural basis for TCR/pMHC and LFA-1/ICAM-1 interandions and 'inside-out' signaling for TCR-mediated LFA-1 activation, via chemical genetics approach [15].
Further refinements could make the assay suitable for high-throughput screening.
Similar(41)
This interference would have to be dealt with, for instance by using adjacent sub-genome specific SNPs to make the assays sub-genome specific.
That trick makes the assay highly versatile.
This modification made the assay less susceptible to artifacts and prevented overestimation of antioxidant power (Huang et al. 2013).
The PCR multiplexes amplify short amplicon lengths which makes the assay sensitive to the analysis of degraded DNA.
A very good leaving group (pKa of DiFMU ∼ 4.7) in the phosphate monoester substrate makes the assay highly sensitive.
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