The very properties that make single-use plastic bags attractive are the same ones that cause environmental harm: they are light, flexible, extremely durable, and, above all, cheap.
That has led many developers, particularly those who make single-use or short-term apps, to worry that they may see an increase in returns, as people "rent" their applications for no cost.
Businesses that make single-use items need to think about what happens to their products when they've reached the end of their lives, said Dune Ives, the head of the environmental nonprofit Lonely Whale Foundation.
A total of 5 µL of 50 ng/ µL genomic DNA was used to make single-use DNA as required by the genotyping system which was arranged in Sentrix Array Matrices (SAMs) each with 96 samples.
The total genomic DNA isolated from fresh bulked leaf tissue of 15 20 plants per genotype was quantified and diluted to 50 ng/μl with TE (10 mM Tris HCl, pH 8.0 and 1 mM EDTA) buffer to make single-use DNA.
Kodak no longer makes refillable film cameras, but still makes single-use film cameras and film; Nikon has discontinued five of its seven film cameras and is phasing out the others.
For the recurrence model, the colonies were isolated and dissociated from three-dimensional lrECM to make single cells using 0.05% trypsin-EDTA, and expanded on two-dimensional plastic on Day 15.
Total RNA (10 μg) was used to make single-stranded cDNA using High Capacity cDNA Reverse Transcription (Applied Biosystems #4368814) per the manufacturer's instructions.
4C-seq and ChIP-seq material was incubated for 45 min with 1 mg/ml collagenase (Sigma-Aldrich, St . Louis MO), and 4C-seq material was further made single cell using a cell strainer (BD Falcon).
Four hundred nanograms of RNA was used to make single-stranded cDNAs (Superscript II; Invitrogen, Karlsruhe, Germany) according to the manufacturer's instructions.
