Existing research [5], [6], [7], [8] on IHC quantification make simplification to the measurement problem by assuming the knowledge of tumour areas and requires manual segmentation of tumour cells.
Alternatively, there are also a number of approaches making simplifications to the reticulate network model, e.g. by imposing additional topological constraints on reticulate networks (Gusfield, 2005; Huson and Klopper, 2007; Huson et al., 2009; Nakhleh et al., 2005) or working with small-scale tree topological features (Huson et al., 2005; Huson and Klopper, 2007, van Iersel et al., 2008).
Although the health states and transitions were designed to mimic real scenarios, the model remains artificial and has to make simplifications, as do all models.
A second tactic is to make simplifications about how species interact.
It is appropriate to make simplifications that allow the expression of a large number of variables with a smaller number of so-called latent variables to determine dependency (correlation) in a multidimensional data set.
In order to construct tractable mathematical descriptions, we must make simplifications of the complicated physics, adopting parameterizations of processes that we feel are important but are unable to describe fully.
For example, while our approach was perceived by some stakeholders as reductionist, the fact needs to be conveyed that all service planning methods make simplifications and assumptions, and are reliant on the accuracy of the available data.
Studies on optimal stand management often make simplifications or restrict the choice of treatments.
Hence, it is imperative to make appropriate simplifications to enable the derivation of analytical solutions that can capture the essential features of repairs.
