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Since mouse models of tuberculosis are usually used as pioneer and proof-of-concept studies to make novel observations regarding infection and immunity, it is not surprising that current understanding of evolution and differentiation of antigen-specific CD4 T cells during M. tuberculosis infection of wild-type hosts is still limited.
Using this information, we make novel observations regarding cancer-normal differences in methylation patterns in various biologically meaningful genome compartments without bias to promoter regions.
Here, we review how the zebrafish– M. marinum system has been deployed to make novel observations about the role of innate immunity, the tuberculous granuloma, and crucial host and bacterial genes.
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Although this study makes novel observations, a limitation to our study is the small number of patients.
While we have recapitulated known biology and made novel observations that are relevant to breast cancer patient management, there are limitations to this study.
Thus, in our validation study using BisPCR, we were able to confirm some previous genome-wide findings and not others, while making novel observations about additional nearby CpGs not covered in prior assays.
With new tools and antibodies for C. elegans MACIT, we have made novel observations on the conservation of functional properties between this MACIT from invertebrates and the mammalian MACIT family.
Planning and interesting ideas clearly helped us make the novel observations above, but we were also aided by good luck and sociology, which I hope continues as our work on this topic progresses.
Our approach allowed us to make several novel observations, such as deducing the potential differences in PPAR signalling and retinol metabolism, as well as the neonatal transcriptomic parallels as the distinguishing features of the MRL/MpJ mouse.
We made three novel observations in cognitively normal individuals.
We made several novel observations in our study.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com