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In the experiments, we make mutations at the nucleotide (rather than amino acid) level, so each point in our sequence space corresponds to a different gene.
Determine DNApol error profile: Assuming the DNApol enzyme is more likely to make mutations at some positions than others, the DNApol error rate for each genome position is also randomly selected from a Beta distribution (αD, βD).
Similarly, for the RT-PCR model, there is again good agreement between the simulated and experimental data sets when one assumes the RT enzyme is more likely to make mutations at some positions than others.
However, when one assumes that the DNApol enzyme is more likely to make mutations at some positions than others, through a Beta distribution, there is good agreement to the experimental data.
Since by definition β≤1, Equation 7 says that cooperative binding can only increase population mean fitness when 2 Nhs < 1 and 2 Ns > 1, i.e if the benefit of cooperativity, h, is sufficient to make mutations at transcription factor binding sites that are deleterious in the absence of cooperativity nearly neutral when it is present.
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The product seen at the sixth A incorporation site must have been generated after IAV Pol made mutations at the five different stop sites prior to the fifth sixth stop site seen in the gel.
We also made mutations at the Q2′ and T6′ positions.
They can dispatch many probes at once and simply make mutations in the genes they want to study.
In my lab, we make mutations all the time and then we change them back.
Make mutation on selected solutions for mutation to get population (P_{1}(T)).
You'd made mutations look so good".
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