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Here, we used a combination of EST sequencing, clustering, annotation of conserved domains and a search for ESTs encoding proteins related to the domain-predicted pathways to make functional predictions of these particular tardigrade-specific EST sequences.
We have designed a series of algorithms that make functional predictions of genes based on orthology and set theory, but our approach to predicting gene function requires no previous knowledge of homolog function.
We highlight the advantages and disadvantages of each type of search, provide examples of large-scale searches of the entire Protein Data Base and make functional predictions.
These data were used by AutoAnnotate to make functional predictions for proteins, which were then made available in the Manatee interface for manual evaluation of the predicted function.
These data used by AutoAnnotate to make functional predictions for proteins were then made available in the Manatee interface for manual evaluation of the predicted function.
In the present paper we do not focus on the network topology or study structural properties thereof but utilize its topology to make functional predictions regarding genes that are cell cycle regulated.
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The DCN of the relevant species was used to make functional prediction for the query protein.
SIFT, Polyphen2, LRT, MutationTaster, and MutationAssessor were used to make functional prediction of missense variants [ 22].
Assessing the MR algorithm on the same target dataset that we used in CSIDOP, MR made functional predictions with an accuracy of 59.50% (Table 1).
SSPs are often highly species-specific, lacking similarity to known proteins, making functional predictions difficult.
While no clear functional prediction can be made for the CHAD, the conserved charged residues could form a strongly polar surface that could participate, either in metal chelation, or act as phospho-acceptors.
More suggestions(15)
make functional changes
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make functional inferences
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make functional telomeres
make poor predictions
make functional connections
make functional objects
make functional absorbents
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