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The copy number of the individual housekeeping gene chosen for relative quantification should be in a similar range to that of the target gene to make comparative quantification possible [ 22].
Selection of either β-actin or PBGD as housekeeping genes was necessary, because the copy number of the housekeeping gene should be in a similar range with that of the target gene to make comparative quantification possible [ 8].
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To make a comparative quantification of the intra-population cellular heterogeneity in gene expression in the Basic and Delay circuit at different time points, we plot in Figure 9 the Coefficient of Variation (CV) of their GFP distributions from Fig. 6 (FACS data).
NG made comparative modeling experiments.
A comparative quantification was made of S100A8 and S100A9 mRNA expression by real time PCR and referred to their respective expression levels by the PDAC metastatic SUIT2 cell line (Table 1).
However, comparative quantification of Ag immersion deposition on bulk Si and PS has not been performed.
Comparative quantification of protein abundances led to the identification of 303 differentially regulated proteins by butanol.
For comparative quantification of phenolic compounds well-established Folin Ciocalteu spectrophotometric method was applied.
The results were analyzed with the software provided with the instrument, using the comparative quantification function.
Comparative quantification of different mRNAs was performed without a normalizer and thus, the 2−ΔΔCt method of quantification [35] was not used.
The expression levels were determined using comparative quantification to the negative control and all quantification data were normalized against 2 reference genes, HMBS and TBP.
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