Exact(1)
What we have done up to this point is to make an alignment of PaxNEB genes from many different animals.
Similar(59)
SIFT is a multistep procedure that (1) searches for and chooses similar sequences (2), makes an alignment of these sequences, and (3) calculates scores based on the amino acids appearing at each position in the alignment.
This test takes all possible triplets of sequences, removes any gaps, and makes an alignment of just the polymorphic sites.
Because of the 100% similarity at the amino acid level between pJAB1 and hJAB1, we next made an alignment of the nucleotide sequences of these two species.
We made an alignment of the Jα helix region from A. thaliana LOV2 with homologous proteins to indicate all mutagenized residues in relation to conserved features.
Five mtDNA sequences representing the three tropical apomictic RKN were obtained and added to the published M. javanica sequence [ 45] making an alignment of 614 bp.
Using Gene Composer software, we made an alignment of each protein with published structural information, as well as the sequences of several homologous proteins for which structural information is not available.
For the MADS-box family, the profile HMM was made from an alignment of all members of this family present in Arabidopsis, excluding sequences that did not align correctly.
The profile HMM for each family was made from an alignment of the conserved region (centred around the DNA binding domain), seeded by protein sequences that were already known to play a role in the flowering time response and other close homologues (see main text for references).
A specific hidden Markov model was made from an alignment of T. gondii ISP1, 2, 3 and 4, and used to search the predicted proteomes of 12 apicomplexan parasites (EuPathDB; http://eupathdb.org) and a further 42 eukaryotes for which complete genome sequence is available (see Wickstead et al., 2010 for full list).
Two modifications should be made: First, an alignment of the P.p Myo8 sequence compared with tobacco (or other solanaceous species) should be included as a supplemental figure and second, changing the phrasing of the last sentence of the paragraph describing the tobacco results to be less emphatic.
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