In this way an active substrate, made up of aggregates of 100 150 nm, was obtained without introducing other interfering material.
Droplets are placed and moved on fibers without touching the glass surface underneath, which is favored by the liquid confinement and hydrophobic character of the bundles, making this material ideal as an active substrate for fluorescence excitation in digital microfluidics.
Human EGF was used as active substrate in ELISA assay.
The substrates tested with these mutants are benzoyl-CoA (highly active substrate for both YbdB and YdiI), 1,4-dihydroxynapthoyl-CoA (highly active substrate for YdiI but not YbdB), and lauroyl-CoA (highly active substrate for YdiI but not YbdB).
Kinase and substrate interact on basis of charge, hydrogen bonding or hydrophobic interactions that make the substrate fit perfectly in the active site [30].
Many SERS-compatible active substrates have been investigated.
These results are in good agreement with the proposed enzyme activity regulation: in the presence of GTP, the catalytic site is shielded by the more compact protein structure, while the conformational changes induced by Ca2+ make the active site accessible to the substrate.
