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We used 200 μl pipette tips to make a straight scratch, simulating a wound.
A sterile pipette tip (200 μl) was used to make a straight scratch on the monolayer of cells and the wound was allowed to heal for 8, 24 and 48 hours.
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After making a straight scratch with a sterile 200 pipette tip, a wound was simulated and cells were treated with high glucose medium (serum free).
This will make a straight line.
Make a straight line like shown.
Make a straight line on your sketches.
Before EGCG treatment, a pipette tip was used to create a straight scratch on the plate to simulate a wound.
Twelve hours after transfection, a straight scratch was made across the cell layer using a 1 ml pipette tip.
Then, a straight scratch was made in individual wells with a 200 μl pipette tip.
Using a 200 μL pipet tip, a straight scratch was made through the cell sheet.
A straight scratch was made gently across the center of the well using a yellow Eppendorf tip.
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