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With the ever-evolving toolset available to the scientific community, the important question one should ask is not simply how to make a mutant line, but rather how best to do so.
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The fact that these processes require a certain progression of larval development in order to be studied makes a mutant line more useful than a morpholino knockdown approach.
We can thus say that the "Hu Line" is also a mutant line of China's minority population.
Standard genetic crosses were used to make double mutant lines of edr2-6 with pad4-1 [ 21], ein2-1 [ 46], coi1-1 [ 47] and the transgene NahG [ 48].
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