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Dr. John J. Mekalanos, a microbiologist at Harvard University, said he had used the cholera genome to make a gene expression chip, a device that tests when any of the bacterium's genes are active.
Who has the right to make a gene?
Furthermore, a lack of consensus in the field as to what attributes make a gene truly essential adds another aspect of variation.
It has been observed that insertion of a transgenic selectable marker to make a gene knockout can influence the expression of neighbouring genes [33].
Obviously the inverse is not true: High expression alone does not make a gene a good target.
In this case, the function of the encoded gene is the feature that would make a gene into a club good.
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There's no way you can make a gene-chip for height.
This product was cloned into pCR2.1 to make a gene-gorging mutagenesis plasmid.
To test whether the V. cholerae repeat is in fact part of an integron, the researchers made a gene "cassette" that contained a Vibrio gene and a marker gene between two copies of the repeat.
Such an "open" chromatin conformation makes a gene susceptible to insertion by transposable elements [ 34].
The specificity of the Cre-lox interaction also provides the basis for making a gene deletion in only certain cells.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com