A total of 86 mutations have been described in the ASPM gene to date that span the entire gene and the majority are predicted to result in protein truncation.
Of the proteins believed to be targeted to the PL NM complex, the majority are predicted to be plastid-targeted.
Satisfyingly, the largest single group of proteins at 22% are annotated in Flybase as 'Plasma membrane', of which a majority are predicted to be integral membrane proteins.
Indeed, 33% of all identified metabolic enzymes are inferred to function in carbohydrate biosynthesis, and the majority are predicted to be localized to the plastid (table 2, supplementary table S1, Supplementary Material online).
The CscA-family members are fairly uniform in size (320 380 residues), and the large majority are predicted to be very basic proteins with a pI above 9.0 (see additional file 2).
Of these, a majority are predicted to be the members of the secretory pathway, while two rice gene products LOC_Os01g56150 and LOC_Os10g36780 are predicted to be mitochondria localised (See additional files 1, 2, 12: Table S1.pdf, Table S2.pdf, Figure SF8.pdf).
An analysis of N-terminal signal sequences of the BAPKO_0422 family of proteins revealed that the majority are predicted to have a functional signal sequence and so may enter the Sec-dependent secretory pathway for translocation across the inner membrane.
Although the majority are predicted to be membrane bound, PSAs predicted to be secreted are found both as strongly conserved proteins between species (PSA21 in all three species; LiPSA12A and LmPSA12A) and as species specific proteins (LiPSA12I and most LmPSA12C).
The majority of fRNAs were predicted in intergenic regions.
SNAP predicted only 1731 (11.3%) of these mutants to be neutral, so the great majority of mutations were predicted to be non-neutral.
