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The majority of weakly EGFP-labeled neurons were identified as MSNs (Fig. 5A, B, type 1), as well as the totality of intense EGFP neurons (Fig. 5A, B, type 2), as revealed by their DARPP-32 immunoreactivity.
Further correlation analyses reveal genome-wide response is biphasic, i) acute-stochastic mode consisting of small number of sharply induced immune-related genes and ii) collective mode consisting of majority of weakly induced genes of diverse cellular processes which collectively adjust their expression level.
High affinity interactions allow relatively stringent wash steps in which the majority of weakly bound proteins are removed.
This is due to the fact that the ranks of the majority of weakly expressed GPCRs vary more or less randomly as their expression is biologically not significant.
We do not present the analyses using the uniform cutoff because this method strongly biases the data; removing the majority of weakly expressed loci compresses the mean expression of the two groups of genes.
Similar(55)
There is a distinction between the base compositional biases in G. lamblia and S. salmonicida; the average G+C content of the S. salmonicida sequences in our dataset is 36%, and the majority of the weakly expressed genes have GC3s values around 20%, while the weakly expressed G. lamblia genes have GC3s around 50% close to the genomic G+C content of G. lamblia [ 60].
However, when the influent was switched to the flowback fluid, the majority of the weakly retained colloids were released, and only a minor portion of those visible at the monitored pore remained retained in the column throughout the flushing event.
However, a separation of the genes into different categories can be observed: the vast majority of the weakly expressed genes have GC3s values between 0.4 and 0.7, while the highly expressed genes have slightly higher values.
Expression levels strongly influence the second axis in the correspondence analysis for S. salmonicida; the highly expressed genes are located at the top separated from the majority of the weakly expressed genes, when axis 2 is plotted against axis 1.
In areas north of California in which field populations are not expected to over-winter, pairwise FST comparisons based on AFLP markers indicate that the majority of populations are weakly, genetically differentiated.
Overall, we concluded that vast majority of transcripts are weakly or not expressed, while only a very small fraction is highly expressed, with no substantial differences between males and females (Pearson's Chi-squared test p-value = 0.21).
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