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The majority of splice variants in PML-RARA, E2A-PBX1, and TEL-AML1 were PML-RARA L-form (50%), E2A-PBX1 (I) (66.7%), and TEL- AML1ex2 (94.4%), respectively.
One other recently developed qPCR primer design software QuantPrime (14) also features gene-specific primer design (i.e. covering the majority of splice variants) as an option.
Truncation of the ORF in the majority of splice variants (particularly MYB) does not necessarily exclude a functional role for these alternative variants.
The majority of splice mutations disrupt the native splice site through a base change within the invariant donor or acceptor dinucleotides (5).
These include the consideration of all gene splice variants to enable either gene-specific (covering the majority of splice variants) or transcript-specific (covering one splice variant) expression profiling, primer specificity validation, automated best primer pair selection according to strict criteria and graphical visualization of the latter primer pairs within their genomic context.
Although the majority of splice variations result in differences between the length of the intracellular loops or the C-terminus, a substantial number results in changes in the length of the N-terminus (as seen for GPR17) and/or sequence (Kilpatrick et al., 1999).
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Recently, a number of studies using global methods have shown that the majority of splicing is co-transcriptional, yet not all published studies agree in their conclusions.
Interestingly, the majority of splicing factors that are alternatively spliced are not differentially expressed (80%).
In agreement with prior observations [28], [49], the majority of splicing factors with significant differential gene expression were up-regulated by activation.
We attempted to identify and quantify the majority of splicing variation within TCF7L2.
Mutation of splice donor sites was undertaken to prevent the majority of splicing within the vector genomic RNA.
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