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The results also showed that the CD44+CD54+ population represents a small fraction of cells in the rectospheres, as the majority of sphere cells did not display CIC properties.
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In the studied biopsies, the majority of spheres were trapped in small arteries with cluster sizes of up to 59 spheres per cluster.
The sphere culture media was changed every 48 h until the majority of spheres reached 100-400 μm in diameter (approximately 7 days).
The majority of spheres settled at a small unknown distance, h o above the coverslip such that Brownian motion was essentially undetectable.
Figure 1C shows that the majority of spheres (64%) are small in diameter (<120 μm), 31% are medium (120-240 μm) and the remaining spheres (5%) are large (>240 μm).
Furthermore, Sox2 staining was detected in the majority of tumor sphere cells isolated from fresh cervical cancer tissues but not from the differentiated cells.
This would be consistent with the results from our study, with the majority of the spheres trapped within cluster sizes of 8to5959 spheres.
The majority of the spheres were found within clusters of two to three spheres, about 30%to40%0% were found to be single spheres, 10% of the spheres were found within clusters of four to five spheres and only a few percent within cluster sizes of seven to ten spheres.
We previously demonstrated that 95% of the spheres in the NSA cannot be passed more than 4 or 6 times suggesting the majority of the spheres are derived from STP cells and that LTP cells exhibit a higher proliferative potential [20], [21].
The threshold level that gave the best quantification of the activity concentration for the majority of the spheres for a given SBR was chosen to be presented in the "Results" section.
The results for the background-adapted threshold that gave the best quantification for the majority of the spheres for a given SBR were chosen to be presented in the "Results" section.
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