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But, for the majority of singletons, they are living alone by choice.
The rationale for this is that the majority of singletons under 100 amino acids in length should be HPs, with many having arisen by chance [100].
A majority of singletons identified were genes not assigned to any COG category.
The majority of singletons (72%) were between 200-350 bp in length, and 4.3% were longer than 400 bp.
The majority of singletons were between 200 bp and 600 bp in length, and 10.3% of them were below 200 bp.
For field pea, the largest proportion of the singletons (21.6%) varied from 301-350 bp, while for faba bean, the majority of singletons (17%) varied from 201-250 bp.
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With the purchase the majority of Singleton's stock, Thomson has a three-newspaper domain in Los Angeles County, with combined total circulation of 124,000.
Unsurprisingly, the majority of singleton ORFs are annotated as HPs, ranging from 68% (R. felis) to 95% (R. typhi) across the analyzed genomes (Figure 10A, B).
Disregarding the gene families under categories of Unknown functions (S) and General function prediction only (R), which include almost one-fourth of the singletons, majority of the singletons (31.6%) appear to be involved in Cell wall/membrane/envelope biogenesis (M), Replication, recombination & repair (L) and Transcription (K) processes (12%, 11.2%, & 8.4% respectively).
The majority of the singletons occupied a distal position in the network, an indication of more recent haplotypes in accordance with the criteria of58.
Almost certainly, the majority of these singleton ORFs are spurious ORFs.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com