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Notably, comprehensive public databases (dbSNP and Ensembl) indicated G as the ancestral, reference allele in the DNA sequence for this locus, but the majority of sequenced samples in our study, 63 out of 67 (or 94%), were homozygous for AA, and only 6% were heterozygous for G (GA).
Authors' response: In the vast majority of sequenced animal genomes, autonomous Transibs are not present at all.
Thus, the vast majority of sequenced high quality bases were target sites, i.e. predicted by the in-silico digest of the orangutan reference genome.
Gene Ontology (GO) analysis of the annotated unigenes revealed that the majority of sequenced genes were associated with metabolic and cellular processes, cell and cell parts, catalytic activity and binding.
Using previously obtained Infinium HumanMethylation450 BeadChip methylation data on sequenced samples [ 19], the Bioconductor package 'DNAcopy' [ 20, 21] was applied to calculate the copy number of the majority of sequenced samples, as described previously [ 22].
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The majority of sequencing errors was identified within homo-nucleotide stretches (112 differences, 66% of all).
However, in lung tumors the majority of sequence variation occurred in the coding region.
The marked genetic heterogeneity, poor functional characterization of the vast majority of sequence variations, and an uncertain genotype-phenotype relationship complicate the definition of mutational search strategies.
The majority of sequences (67.2% CD and 43.3% non-IBD) were found in the OTU 1.
These sequences were almost entirely derived from fish, with the majority of sequences matching the myctophid fish K. andersoni.
The second cluster contained two specimens and the majority of sequences in both of these were Lactobacillus iners.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com