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Analysis of the circulating toxin components revealed that the majority of detectable PA exists as the proteolytically-processed PA63 form, which is either assembled or capable of assembling with LF in a manner analogous to what is observed on cell surfaces [27], [28], [29].
Nevertheless, as described in yeast, the majority of detectable regional gene expression biases in these mammalian tissues also coincide with chromosomal amplifications or deletions.
This indicates that the majority of detectable tissue-specific sequence signals reside within proximal promoters while signals outside of this well-defined region get overshadowed by sequence noise.
Comparison of pY protein levels in different membrane fractions and cytosolic protein (S100) indicated that the vast majority of detectable pY proteins is found in the S100 (data not shown) and this fraction was further investigated.
First, we see that the overwhelming majority of detectable GRs and IRs, along with at least half of the detectable ORs consist of genes that have clear orthologs in the transcriptomes of all three taxa.
In addition, ORs with dN/dS ratios above the transcriptome median (0.0611) comprised the majority of detectable ORs and showed significantly higher levels of enrichment than those genes in the transcriptome background in the upper half of the dN/dS (Wilcoxon rank sum test, p=0.02792).
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538 of the 696 total phosphoproteins (>77%) identified in the CLL A data sets were also identified in data sets B - E, suggesting detection of the majority of phosphopeptides detectable by these methods.
The observed larger MPs were in fact MP-containing ICs (mpICs) and account for the majority of the detectable ICs.
Of note, increased proinflammatory and decreased anti-inflammatory markers account for the majority of changes detectable in a large panel of cerebrospinal fluid analytes in MCI and AD [ 49, 50].
The majority of the detectable Tregs in islets are phenotypically CD4+Foxp3+Bcl-2loKi67+, which may represent newly differentiated iTregs at the site of inflammation.
Our findings refine and unify these discrepant observations by demonstrating that although only a proportion (∼7%) of the MPs are part of mpICs, the majority of the detectable ICs are in fact mpICs.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com