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However, the cleavage pattern over centromeres is different from the large majority of cleavages on chromosome arms.
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Executioner caspase-3 is responsible for the majority of cleavage events and its therapeutic potential is of high interest with to date several available active site peptide inhibitors.
The predicted tryptic map for the human PrP sequence shows that the majority of cleavage sites are within the PK-resistant core (Fig. 1A, gray triangles) with a few additional cleavage sites between residues 23 49 of the mature PrP sequence (Fig. 1A, red triangles) and no sites within the octarepeat region (residues 51 91).
Similarities between guinea pig and human ASNase3 cleavage rates were also observed in the presence of 250 mM glycine with the majority of cleavage occurring within 24 h.
Although caspases and calpains are reported to account for the majority of cleavage effects on polyQ expanded disease proteins, several fragmentation events could not be explained by their proteolytic activity.
CBHs catalyse the majority of bond cleavages during cellulose hydrolysis and are usually the major component of fungus-derived commercial cellulase mixtures [ 13] or artificial enzyme mixtures for cellulose hydrolysis [ 14– 20].
The virus genome is translated to produce a single polypeptide chain that subsequently is cleaved by viral proteases into mature protein products, with one protease, 3Cpro, carrying out the majority of the cleavages.
Caspase 3 is an effector caspase that performs the majority of protein cleavages associated with apoptosis.
Interestingly the majority of the cleavages of the hydrophobic amino acids took place at the NH2-group of the amino acid.
Although some moderate (10 30%) and extreme (>80%) cleavage yields may be a consequence of the assumptions made for the calculation (see Materials and Methods), the majority of the cleavage yields can be correlated to the secondary structure at the cleavage site, as suggested earlier [8], regardless of the scale of the purification.
Majority of the cleavage sites (90.7%) had an rSASA value >0.4.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com