These observations showed that the majority of aggregates are charged.
During heparin chromatography, the correctly folded dimer eluted at 200 mM NaCl (H1) and the majority of aggregates as a large peak at 600 mM NaCl (H2) (Fig. 2B and Fig. S2A).
Even with the harshest pretreatment, the majority of aggregates get stuck at the bottom of the loading pocket of the gel for electrophoretic separation.
As shown in Figure 3 (also see Table S3) the majority of aggregates formed in Cultisher-S cultures after 24 h were <49 µm in diameter, irrespective of seeding parameters.
Under nutritional stress, the majority of bacteria aggregate to form a bulging mass called fruiting body while a small number of vegetative cells (about 10%), named peripheral rods, remain undifferentiated and are located around and between fruiting bodies.
Native MstnPP was purified by heparin affinity chromatography that separated the correctly folded MstnPP dimer from the majority of the aggregates followed by the removal of remaining aggregates by gel filtration.
While several of these elongated aggregates of Italian Aβ (1 40) appeared rigid and lacking discernable curvature, the majority of fibrillar aggregates formed by Italian Aβ (1 40) had curled morphology reminiscent of the elongated aggregates observed for Arctic Aβ (1 40).
Some cellular aggregates of H1650 cells were observed after 48 h, but the vast majority of these aggregates collapsed and disintegrated within a few days.
First, the Mori–Tanaka method is utilized to include the majority of small aggregates and air voids.
