A total of 38 major HpSVd variants were identified and aligned to detect natural sequence changes associated with grapevines cultivated in Japan (Supp. Table S1): major variations were present at positions 26, 32, 44/45, 46, 47, 180, 256 and 257 (Fig. 1B).
After the radiotelemetry transmitter implantation, the dogs were rested for at least 5 weeks, until no major variations were identified in echocardiographic evaluation and LAP.
Further analysis demonstrated that the increase occurred mostly in primary care, and major variations were noted among municipalities as well as among healthcare centres (local prescription data).
Amplification at the SUL1 locus was not detected by qPCR, and the absence of SUL1 amplification or other major variations was confirmed by aCGH (Table 4 and Figure S5).
Whereas it is possible that the presence of the researchers during health education talks could have affected the quality and length of the talks, no major variations were observed in talks done at the start of the study and those at the end.
It was shown that major variations were located in NSP2-coding regincludingding 3-aa deletion at the position aa 593 595 in GZ1101, 18-aa deletion at the position aa 482 499 in LN1101, 30-aa deletion at the positions aa 482 and aa 533 561 in BJ1102, and 31-aa deletion at the positions aa 468, aa 482, and aa 533 561 and an amino acid "P" insertion between aa 585 and aa 586 in SD0901.
No major variation was observed among biological replicates of each variety as most of the features were falling along the diagonal.
Among biological replicates of each cultivar under MV or mock treatment, nearly all of the probe sets fell along the diagonal, which indicated that no major variation was observed.
Major variation was exhibited among the subfamilies and species.
While actual content varied little, the major variation was found in the method and rigor of guideline development as well as the comprehensiveness [ 5- 8].
