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Two major transcripts were observed in the tumors: one 2.7 kb and one 6.5 kb.
Likewise, only introns of major transcripts were selected.
Two major transcripts were identified: type I encodes a protein of 647 amino acids and type II encodes a protein of 1365 amino acids.
Under our conditions, these two major transcripts were detected only in Bp-ALL (NALM6 and REH) whereas in T-ALL (CCRF-CEM) cells only the longer transcript was detected.
The sequence alignments of the major transcripts were illustrated in the following figures according to their respective toxin families: 3FTxs (Fig. 5), SVMPs (Fig. 7), CRISPs (Fig. 8), LAAOs (Fig. 9), vespryns (Fig. 10), PLA2s (Fig. 11) and CVFs (Fig. 12).
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Second, one or two major transcripts are most often observed, which would suggest either that only one or two major transcription start sites exist, or that if many transcription start sites are utilised then these are either very close together or else only one or two give rise to a major stable transcript.
Four major transcripts are produced from the same gene.
If there are multiple transcripts for a gene, the transcription start site (TSS) and 3' UTR of the major transcript were used [ 23].
The larger 2.6 kb major transcript was identical in size to Lim3A; the minor 2.4 kb transcript, which we called Lim3C, was new.
Whenever multiple spliced transcripts were available for a gene, a major transcript was chosen (Atngnnnnn.1) to prevent bias towards genes that contain multiple transcripts.
The brain-specific transcripts therefore show paternal expression, and the maternal expression of the major transcript is secondarily regulated by the differential methylation of the downstream CGI (Shiura et al. 2009).
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